Super-Resolution Fingerprinting Detects Chemical Reactions and Idiosyncrasies of Single DNA Pegboards
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Author(s)
Johnson-Buck, Alexander
Nangreave, Jeanette
Kim, Do-Nyun
Bathe, Mark
Yan, Hao
Walter, Nils G.
Date Issued
January 2013
Journal
Nano Letters
Publisher
American Chemical Society (ACS)
Citation
Johnson-Buck, Alexander, Jeanette Nangreave, Do-Nyun Kim, Mark Bathe, Hao Yan, and Nils G. Walter. “Super-Resolution Fingerprinting Detects Chemical Reactions and Idiosyncrasies of Single DNA Pegboards.” Nano Lett. 13, no. 2 (February 13, 2013): 728–733.
Version
Original manuscript
Abstract
We employ the single-particle fluorescence nanoscopy technique points accumulation for imaging in nanoscale topography (PAINT) using site-specific DNA probes to acquire two-dimensional density maps of specific features patterned on nanoscale DNA origami pegboards. We show that PAINT has a localization accuracy of ∼10 nm that is sufficient to reliably distinguish dense (>10[superscript 4] features μm[superscript –2]) sub-100 nm patterns of oligonucleotide features. We employ two-color PAINT to follow enzyme-catalyzed modification of features on individual origami and to show that single nanopegboards exhibit stable, spatially heterogeneous probe-binding patterns, or “fingerprints.” Finally, we present experimental and modeling evidence suggesting that these fingerprints may arise from feature spacing variations that locally modulate the probe binding kinetics. Our study highlights the power of fluorescence nanoscopy to perform quality control on individual soft nanodevices that interact with and position reagents in solution.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1021/nl304415b
