Multi-Material Tissue Engineering Scaffold with Hierarchical Pore Architecture
Name
nihms833016.pdf
Description
Accepted version
Size
2.51 MB
Format
Adobe PDF
Checksum (MD5)
1887350b5f58b3b16278ce850460a620
Author(s)
Morgan, Kathy Ye
Sklaviadis, Demetra
Tochka, Zachary L
Fischer, Kristin M
Hearon, Keith
Morgan, Thomas D
Langer, Robert
Freed, Lisa E
Date Issued
2016
Journal
Advanced Functional Materials
Publisher
Wiley
Citation
Morgan, Kathy Ye, et al. "Multi-Material Tissue Engineering Scaffold with Hierarchical Pore Architecture." Advanced Functional Materials 26 32 (2016): 5873-83.
Version
Author's final manuscript
Abstract
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Multi-material polymer scaffolds with multiscale pore architectures are characterized and tested with vascular and heart cells as part of a platform for replacing damaged heart muscle. Vascular and muscle scaffolds are constructed from a new material, poly(limonene thioether) (PLT32i), which meets the design criteria of slow biodegradability, elastomeric mechanical properties, and facile processing. The vascular–parenchymal interface is a poly(glycerol sebacate) (PGS) porous membrane that meets different criteria of rapid biodegradability, high oxygen permeance, and high porosity. A hierarchical architecture of primary (macroscale) and secondary (microscale) pores is created by casting the PLT32i prepolymer onto sintered spheres of poly(methyl methacrylate) (PMMA) within precisely patterned molds followed by photocuring, de-molding, and leaching out the PMMA. Prefabricated polymer templates are cellularized, assembled, and perfused in order to engineer spatially organized, contractile heart tissue. Structural and functional analyses show that the primary pores guide heart cell alignment and enable robust perfusion while the secondary pores increase heart cell retention and reduce polymer volume fraction.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
10.1002/ADFM.201601146
https://doi.org/10.1002/ADFM.201601146
