Proteomic Mapping of the Human Mitochondrial Intermembrane Space in Live Cells via Ratiometric APEX Tagging
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Ting_Proteomic Mapping.pdf
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Author(s)
Udeshi, Namrata D.
Cracan, Valentin
Svinkina, Tanya
Hung, Victoria
Zou, Peng
Rhee, Hyun-Woo
Carr, Steven A
Mootha, Vamsi
Ting, Alice Y
Date Issued
July 2014
Journal
Molecular Cell
Publisher
Elsevier
Citation
Hung, Victoria; Zou, Peng; Rhee, Hyun-Woo; Udeshi, Namrata D.; Cracan, Valentin; Svinkina, Tanya; Carr, Steven A.; Mootha, Vamsi K. and Ting, Alice Y. "Proteomic Mapping of the Human Mitochondrial Intermembrane Space in Live Cells via Ratiometric APEX Tagging." Molecular Cell 55, no. 2: 332-341 © 2014 Elsevier Inc
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Author's final manuscript
Abstract
Obtaining complete protein inventories for subcellular regions is a challenge that often limits our understanding of cellular function, especially for regions that are impossible to purify and are therefore inaccessible to traditional proteomic analysis. We recently developed a method to map proteomes in living cells with an engineered peroxidase (APEX) that bypasses the need for organellar purification when applied to membrane-bound compartments; however, it was insufficiently specific when applied to unbounded regions that allow APEX-generated radicals to escape. Here, we combine APEX technology with a SILAC-based ratiometric tagging strategy to substantially reduce unwanted background and achieve nanometer spatial resolution. This is applied to map the proteome of the mitochondrial intermembrane space (IMS), which can freely exchange small molecules with the cytosol. Our IMS proteome of 127 proteins has >94% specificity and includes nine newly discovered mitochondrial proteins. This approach will enable scientists to map proteomes of cellular regions that were previously inaccessible.
MIT Department
Broad Institute of MIT and Harvard
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2014.06.003
