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Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging
Name
pbio.3000965.pdf
Description
Published version
Size
2.36 MB
Format
Adobe PDF
Checksum (MD5)
63a40d7c5a9b758bcf0666b9114a7a64
Author(s)
Qian, Yong
Cosio, Danielle M Orozco
Piatkevich, Kiryl D
Aufmkolk, Sarah
Su, Wan-Chi
Celiker, Orhan T
Schohl, Anne
Murdock, Mitchell H
Aggarwal, Abhi
Chang, Yu-Fen
Date Issued
2020
Journal
PLoS Biology
Publisher
Public Library of Science (PLoS)
Version
Final published version
Abstract
© 2020 Qian et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
10.1371/journal.pbio.3000965
