A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells
Name
fphar-11-00453.pdf
Description
Published version
Size
2.09 MB
Format
Adobe PDF
Checksum (MD5)
81987d73bc2e98445ff33cf944c1dc3a
Author(s)
Bai, Jing
Fu, Haojie
Bazinet, Lauren
Birsner, Amy E.
D'Amato, Robert J.
Date Issued
April 2020
Journal
Frontiers in Pharmacology
Publisher
Frontiers Media SA
Citation
Bai, Jing et al. "A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells." Frontiers in Pharmacology 11 (April 2020): 453
Version
Final published version
Abstract
Microfluidic-based organ-on-a-chip assays with simultaneous coculture of multi-cell types have been widely utilized for basic research and drug development. Here we describe a novel method for a primary cell-based corneal microphysiological system which aims to recapitulate the basic functions of the in vivo cornea and to study topically applied ocular drug permeation. In this study, the protocols for isolating and cultivating primary corneal epithelial cells and endothelial cells from mouse inbred strain C57BL/6J were optimized, to allow for the development of a primary-cell based microfluidic 3D micro-engineered cornea. This tissue unit, by overcoming the limitations of 2D conventional cell culture, supports new investigations on cornea function and facilitates drug delivery testing.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3389/fphar.2020.00453
