In Vivo Label-Free Quantification of Liver Microcirculation Using Dual-Modality Microscopy
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Yan-2014-In vivo label.pdf
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2.95 MB
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Checksum (MD5)
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Author(s)
Yan, Jie
Kang, Yuzhan
Xu, Shuoyu
Ong, Lee Ling
Zhuo, Shuangmu
Bunte, Ralph M.
Chen, Nanguang
Wanless, Ian R.
Yu, Hanry
Asada, Harry
Date Issued
November 2014
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Yan, Jie, Yuzhan Kang, Shuoyu Xu, Lee-Ling S. Ong, Shuangmu Zhuo, Ralph M Bunte, Nanguang Chen, et al. “ In Vivo Label-Free Quantification of Liver Microcirculation Using Dual-Modality Microscopy .” Journal of Biomedical Optics 19, no. 11 (November 1, 2014): 116006. © 2014 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progression of liver disease. We have combined wide-field fluorescence microscopy and a laser speckle contrast technique to characterize hepatic microcirculation in vivo without labeling in a bile-duct ligation rat fibrosis model of biliary atresia. Through quantitative image analysis of four microcirculation parameters, we observed significant microcirculation lesion in the early to middle stages of fibrosis. This bimodal imaging method is useful to assess hepatic microcirculation lesion for the study of liver diseases.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.jbo.19.11.116006
