A robust cell culture system supporting the complete life cycle of hepatitis B virus
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Author(s)
Michailidis, Eleftherios
Pabon, Jonathan
Xiang, Kuanhui
Park, Paul
Ramanan, Vyas
Hoffmann, Hans-Heinrich
Schneider, William M.
de Jong, Ype P.
Shlomai, Amir
Rice, Charles M.
Date Issued
November 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Michailidis, Eleftherios et al. “A Robust Cell Culture System Supporting the Complete Life Cycle of Hepatitis B Virus.” Scientific Reports 7, 1 (November 2017): 16616 © 2017 The Author(s)
Version
Final published version
Abstract
The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the hepatitis B virus (HBV) receptor enabled researchers to create hepatoma cell lines susceptible to HBV infection. Infection in current systems, however, is inefficient and virus fails to spread. Infection efficiency is enhanced by treating cells with polyethylene glycol 8000 (PEG) during infection. However, this alone does not promote virus spread. Here we show that maintaining PEG in culture medium increases the rate of infection by at least one order of magnitude, and, most importantly, promotes virus spread. To demonstrate the utility of this system, we show that two interferon-stimulated genes (ISGs), ISG20 and tetherin, restrict HBV spread in NTCP-expressing hepatoma cells. Thus, this protocol can be easily applied to existing cell culture systems to study the complete HBV life cycle, including virus spread.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/S41598-017-16882-5
