Ascites analysis by a microfluidic chip allows tumor-cell profiling
Name
Peterson-2013-Ascites analysis by.pdf
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1.87 MB
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Checksum (MD5)
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Author(s)
Peterson, Vanessa M.
Castro, Cesar M.
Chung, Jaehoon
Miller, Nathan C.
Ullal, Adeeti V.
Castano, Maria D.
Penson, Richard T.
Lee, Hakho
Birrer, Michael J.
Weissleder, Ralph
Date Issued
December 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Peterson, V. M., C. M. Castro, J. Chung, N. C. Miller, A. V. Ullal, M. D. Castano, R. T. Penson, H. Lee, M. J. Birrer, and R. Weissleder. “Ascites Analysis by a Microfluidic Chip Allows Tumor-Cell Profiling.” Proceedings of the National Academy of Sciences 110, no. 51 (December 2, 2013): E4978–E4986.
Version
Final published version
Abstract
Ascites tumor cells (ATCs) represent a potentially valuable source of cells for monitoring treatment of ovarian cancer as it would obviate the need for more invasive surgical biopsies. The ability to perform longitudinal testing of ascites in a point-of-care setting could significantly impact clinical trials, drug development, and clinical care. Here, we developed a microfluidic chip platform to enrich ATCs from highly heterogeneous peritoneal fluid and then perform molecular analyses on these cells. We evaluated 85 putative ovarian cancer protein markers and found that nearly two-thirds were either nonspecific for malignant disease or had low abundance. Using four of the most promising markers, we prospectively studied 47 patients (33 ovarian cancer and 14 control). We show that a marker set (ATC[subscript dx]) can sensitively and specifically map ATC numbers and, through its reliable enrichment, facilitate additional treatment-response measurements related to proliferation, protein translation, or pathway inhibition.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1315370110
