Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
Name
s41467-020-19097-x.pdf
Description
Published version
Size
1016.72 KB
Format
Adobe PDF
Checksum (MD5)
d395fc08352b690a464cd2c4d72d4375
Author(s)
Arizti-Sanz, Jon
Freije, Catherine A.
Stanton, Alexandra C.
Petros, Brittany A.
Boehm, Chloe K.
Siddiqui, Sameed
Shaw, Bennett M.
Adams, Gordon
Kosoko-Thoroddsen, Tinna-Solveig F.
Kemball, Molly E.
Date Issued
November 2020
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Arizti-Sanz, Jon et al. "Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2." Nature Communications 11, 1 (November 2020): 5921 © 2020 The Author(s)
Version
Final published version
Abstract
The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE’s results can be visualized with an in-tube fluorescent readout — reducing contamination risk as amplification reaction tubes remain sealed — and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41467-020-19097-x
