Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device
Name
acs.analchem.9b05043.pdf
Description
Published version
Size
2.41 MB
Format
Adobe PDF
Checksum (MD5)
9386093e3c64882ba3453ea68ba1bb1f
Author(s)
Lee, Hyewon
Lee, Jiseok
Doyle, Patrick S
Date Issued
March 2020
Journal
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Citation
Lee, Hyewon et al. “Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device” Analytical Chemistry, vol. 92, no. 8, 2020, pp. 5750-5755 © 2020 The Author(s)
Version
Final published version
Abstract
Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling of miRNAs, without the use of complex external equipment for fluidics and imaging. For sensitive and reliable measurement without the risk of sequence bias, we employed a gold deposition-based signal amplification scheme and dark-field imaging, and seamlessly integrated a previously developed miRNA assay scheme into this platform. The assay demonstrated a limit of detection of 260 fM, along with multiplexing of small panels of miRNAs in healthy and cancer samples. We anticipate this versatile platform to facilitate a broad range of POC profiling of miRNAs in cancer-associated dysregulation with high-confidence by exploiting the unique features of hydrogel substrate in an on-chip format and colorimetric analysis.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/acs.analchem.9b05043
