Systems analysis of the CO[subscript 2] concentrating mechanism in cyanobacteria
Name
Mangan-2014-Systems analysis of.pdf
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1.87 MB
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3aba2b6fb3fc73bcbdba165962863843
Author(s)
Mangan, Niall Mari
Brenner, Michael P.
Alternative Title
Systems analysis of the CO2 concentrating mechanism in cyanobacteria
Date Issued
April 2014
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Mangan, Niall M, and Michael P Brenner. “ Systems Analysis of the CO 2 Concentrating Mechanism in Cyanobacteria .” Abstract 3 (April 29, 2014).
Version
Final published version
Abstract
Cyanobacteria are photosynthetic bacteria with a unique CO2 concentrating mechanism (CCM), enhancing carbon fixation. Understanding the CCM requires a systems level perspective of how molecular components work together to enhance CO2 fixation. We present a mathematical model of the cyanobacterial CCM, giving the parameter regime (expression levels, catalytic rates, permeability of carboxysome shell) for efficient carbon fixation. Efficiency requires saturating the RuBisCO reaction, staying below saturation for carbonic anhydrase, and avoiding wasteful oxygenation reactions. We find selectivity at the carboxysome shell is not necessary; there is an optimal non-specific carboxysome shell permeability. We compare the efficacy of facilitated CO2 uptake, CO2 scavenging, and HCO3− transport with varying external pH. At the optimal carboxysome permeability, contributions from CO2 scavenging at the cell membrane are small. We examine the cumulative benefits of CCM spatial organization strategies: enzyme co-localization and compartmentalization.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.7554/eLife.02043
