Arp2/3 Is a Negative Regulator of Growth Cone Translocation
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Gertler_Arp2.pdf
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Author(s)
Rahim, Nazimah Abdul
VanderWaal, Kristyn E
Lanier, Lorene M.
Strasser, Geraldine A.
Gertler, Frank
Date Issued
July 2004
Journal
Neuron
Publisher
Elsevier
Citation
Strasser, Geraldine A, Nazimah Abdul Rahim, Kristyn E VanderWaal, Frank B Gertler, and Lorene M Lanier. “Arp2/3 Is a Negative Regulator of Growth Cone Translocation.” Neuron 43, no. 1 (July 8, 2004): 81-94. Copyright © 2004 Cell Press
Version
Final published version
Abstract
Arp2/3 is an actin binding complex that is enriched in the peripheral lamellipodia of fibroblasts, where it forms a network of short, branched actin filaments, generating the protrusive force that extends lamellipodia and drives fibroblast motility. Although it has been assumed that Arp2/3 would play a similar role in growth cones, our studies indicate that Arp2/3 is enriched in the central, not the peripheral, region of growth cones and that the growth cone periphery contains few branched actin filaments. Arp2/3 inhibition in fibroblasts severely disrupts actin organization and membrane protrusion. In contrast, Arp2/3 inhibition in growth cones minimally affects actin organization and does not inhibit lamellipodia protrusion or de novo filopodia formation. Surprisingly, Arp2/3 inhibition significantly enhances axon elongation and causes defects in growth cone guidance. These results indicate that Arp2/3 is a negative regulator of growth cone translocation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.neuron.2004.05.015
