Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls
Name
Barker-2012-Identifying the Stem.pdf
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1.01 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Barker, Nick
van Oudenaarden, Alexander
Clevers, Hans
van Oudenaarden, Alexander
Date Issued
October 2012
Journal
Cell Stem Cell
Publisher
Elsevier
Citation
Barker, Nick, Alexander van Oudenaarden, and Hans Clevers. “Identifying the Stem Cell of the Intestinal Crypt: Strategies and Pitfalls.” Cell Stem Cell 11, no. 4 (October 2012): 452–460. © 2012 Elsevier Inc.
Version
Final published version
Abstract
Decades ago, two nonoverlapping crypt stem cell populations were proposed: Leblond’s Crypt Base Columnar (CBC) cell and Potten’s +4 cell. The identification of CBC markers including Lgr5 has confirmed Leblond’s predictions that CBC cells are anatomically distinct, long-lived stem cells that permanently cycle. While Potten originally described +4 cells as proliferative and unusually radiation-sensitive, recent efforts to identify +4 stem cells have focused on the identification of cells that are quiescent and radiation-resistant. Here, we describe commonalities and discrepancies between the individual studies and discuss challenges of marker-based lineage tracing.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Physics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.stem.2012.09.009
