Single-wavelength reflected confocal and multiphoton microscopy for tissue imaging
Name
Chen-2009-Single-wavelength re.pdf
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1.34 MB
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Checksum (MD5)
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Author(s)
So, Peter T. C.
Kim, Daekeun
Kim, Ki Hean
Dong, Chen-Yuan
Lin, Sung-Jan
Tsai, Tsung-Hua
Tan, Hsin-Yuan
Jee, Shiou-Hwa
Chen, Yang-Fang
Lin, Ming-Gu
Date Issued
October 2009
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-Optical Instrumentation Engineers
Citation
Chen, Wei-Liang et al. “Single-wavelength reflected confocal and multiphoton microscopy for tissue imaging.” Journal of Biomedical Optics 14.5 (2009): 054026-8. © 2009 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Both reflected confocal and multiphoton microscopy can have clinical diagnostic applications. The successful combination of both modalities in tissue imaging enables unique image contrast to be achieved, especially if a single laser excitation wavelength is used. We apply this approach for skin and corneal imaging using the 780-nm output of a femtosecond, titanium-sapphire laser. We find that the near-IR, reflected confocal (RC) signal is useful in characterizing refractive index varying boundaries in bovine cornea and porcine skin, while the multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) intensities can be used to image cytoplasm and connective tissues (collagen), respectively. In addition, quantitative analysis shows that we are able to detect MAF from greater imaging depths than with the near-IR RC signal. Furthermore, by performing RC imaging at 488, 543, and 633 nm, we find that a longer wavelength leads to better image contrast for deeper imaging of the bovine cornea and porcine skin tissue. Finally, by varying power of the 780-nm source, we find that comparable RC image quality was achieved in the 2.7 to 10.7-mW range.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1117/1.3247157
