New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay
Name
Adv Healthcare Materials - 2022 - Wan - New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic.pdf
Size
7.2 MB
Format
Adobe PDF
Checksum (MD5)
f2865e40d68f4ec088f0430e3c99a626
Author(s)
Wan, Zhengpeng
Floryan, Marie A
Coughlin, Mark F
Zhang, Shun
Zhong, Amy X
Shelton, Sarah E
Wang, Xun
Xu, Chenguang
Barbie, David A
Kamm, Roger D
Date Issued
November 4, 2022
Journal
Advanced Healthcare Materials
Publisher
Wiley
Citation
Wan, Zhengpeng, Floryan, Marie A, Coughlin, Mark F, Zhang, Shun, Zhong, Amy X et al. 2022. "New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay." Advanced Healthcare Materials.
Version
Final published version
Abstract
Previous studies have developed vascularized tumor spheroid models to demonstrate the impact of intravascular flow on tumor progression and treatment. However, these models have not been widely adopted so the vascularization of tumor spheroids in vitro is generally lower than vascularized tumor tissues in vivo. To improve the tumor vascularization level, a new strategy is introduced to form tumor spheroids by adding fibroblasts (FBs) sequentially to a pre-formed tumor spheroid and demonstrate this method with tumor cell lines from kidney, lung, and ovary cancer. Tumor spheroids made with the new strategy have higher FB densities on the periphery of the tumor spheroid, which tend to enhance vascularization. The vessels close to the tumor spheroid made with this new strategy are more perfusable than the ones made with other methods. Finally, chimeric antigen receptor (CAR) T cells are perfused under continuous flow into vascularized tumor spheroids to demonstrate immunotherapy evaluation using vascularized tumor-on-a-chip model. This new strategy for establishing tumor spheroids leads to increased vascularization in vitro, allowing for the examination of immune, endothelial, stromal, and tumor cell responses under static or flow conditions.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
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DOI of Published Version
https://doi.org/10.1002/adhm.202201784
