Influenza virus exploits tunneling nanotubes for cell-to-cell spread
Name
Influenza virus exploits.pdf
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1.79 MB
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Checksum (MD5)
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Author(s)
Kumar, Amrita
Kim, Jin Hyang
Ranjan, Priya
Metcalfe, Maureen G.
Cao, Weiping
Mishina, Margarita
Gangappa, Shivaprakash
Guo, Zhu
Zaki, Sherif
York, Ian
Date Issued
January 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Kumar, Amrita, Jin Hyang Kim, Priya Ranjan, Maureen G. Metcalfe, Weiping Cao, Margarita Mishina, Shivaprakash Gangappa, et al. “Influenza Virus Exploits Tunneling Nanotubes for Cell-to-Cell Spread.” Scientific Reports 7 (January 6, 2017): 40360.
Version
Final published version
Abstract
Tunneling nanotubes (TNTs) represent a novel route of intercellular communication. While previous work has shown that TNTs facilitate the exchange of viral or prion proteins from infected to naïve cells, it is not clear whether the viral genome is also transferred via this mechanism and further, whether transfer via this route can result in productive replication of the infectious agents in the recipient cell. Here we present evidence that lung epithelial cells are connected by TNTs, and in spite of the presence of neutralizing antibodies and an antiviral agent, Oseltamivir, influenza virus can exploit these networks to transfer viral proteins and genome from the infected to naïve cell, resulting in productive viral replication in the naïve cells. These observations indicate that influenza viruses can spread using these intercellular networks that connect epithelial cells, evading immune and antiviral defenses and provide an explanation for the incidence of influenza infections even in influenza-immune individuals and vaccine failures.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Mathematics
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Media Laboratory
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/srep40360
