Enteric YaiW Is a Surface-Exposed Outer Membrane Lipoprotein That Affects Sensitivity to an Antimicrobial Peptide
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Arnold et al JB Final Submit.pdf
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Author(s)
Walker, Graham C.
Arnold, Markus F. F.
Caro-Hernandez, Paola
Tan, Karen
Runti, Giulia
Wehmeier, Silvia
Scocchi, Marco
Doerrler, William T.
Ferguson, Gail P.
Date Issued
November 2013
Journal
Journal of Bacteriology
Publisher
American Society for Microbiology
Citation
Arnold, M. F. F., P. Caro-Hernandez, K. Tan, G. Runti, S. Wehmeier, M. Scocchi, W. T. Doerrler, G. C. Walker, and G. P. Ferguson. “Enteric YaiW Is a Surface-Exposed Outer Membrane Lipoprotein That Affects Sensitivity to an Antimicrobial Peptide.” Journal of Bacteriology 196, no. 2 (January 15, 2014): 436–444.
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Author's final manuscript
Abstract
yaiW is a previously uncharacterized gene found in enteric bacteria that is of particular interest because it is located adjacent to the sbmA gene, whose bacA ortholog is required for Sinorhizobium meliloti symbiosis and Brucella abortus pathogenesis. We show that yaiW is cotranscribed with sbmA in Escherichia coli and Salmonella enterica serovar Typhi and Typhimurium strains. We present evidence that the YaiW is a palmitate-modified surface exposed outer membrane lipoprotein. Since BacA function affects the very-long-chain fatty acid (VLCFA) modification of S. meliloti and B. abortus lipid A, we tested whether SbmA function might affect either the fatty acid modification of the YaiW lipoprotein or the fatty acid modification of enteric lipid A but found that it did not. Interestingly, we did observe that E. coli SbmA suppresses deficiencies in the VLCFA modification of the lipopolysaccharide of an S. meliloti bacA mutant despite the absence of VLCFA in E. coli. Finally, we found that both YaiW and SbmA positively affect the uptake of proline-rich Bac7 peptides, suggesting a possible connection between their cellular functions.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1128/JB.01179-13
