Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
Name
nihms-1533068.pdf
Description
Accepted version
Size
4.27 MB
Format
Adobe PDF
Checksum (MD5)
39378a2ef2b169ed5ce257de2bda7677
Author(s)
Saka, Sinem K
Wang, Yu
Kishi, Jocelyn Y
Zhu, Allen
Zeng, Yitian
Xie, Wenxin
Kirli, Koray
Yapp, Clarence
Cicconet, Marcelo
Beliveau, Brian J
Date Issued
2019
Journal
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Version
Author's final manuscript
Abstract
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. Spatial mapping of proteins in tissues is hindered by limitations in multiplexing, sensitivity and throughput. Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated by primer exchange reaction (PER). SABER offers independently programmable signal amplification without in situ enzymatic reactions, and intrinsic scalability to rapidly amplify and visualize a large number of targets when combined with fast exchange cycles of fluorescent imager strands. We demonstrate 5- to 180-fold signal amplification in diverse samples (cultured cells, cryosections, formalin-fixed paraffin-embedded sections and whole-mount tissues), as well as simultaneous signal amplification for ten different proteins using standard equipment and workflows. We also combined SABER with expansion microscopy to enable rapid, multiplexed super-resolution tissue imaging. Immuno-SABER presents an effective and accessible platform for multiplexed and amplified imaging of proteins with high sensitivity and throughput.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/S41587-019-0207-Y
