Molecular movement in the Arabidopsis thaliana female gametophyte
Name
Erdmann2017_Article_MolecularMovementInTheArabidop.pdf
Description
Published version
Size
3.19 MB
Format
Adobe PDF
Checksum (MD5)
3ed9deded16ab00a25cb90604eb04c44
Author(s)
Erdmann, Robert Michael
Hoffmann, Anja
Walter, Heidi-Kristin
Wagenknecht, Hans-Achim
Groß-Hardt, Rita
Gehring, Mary
Date Issued
July 2017
Journal
Plant reproduction
Publisher
Springer Science and Business Media LLC
Citation
Erdmann, Robert M. et al. "Molecular movement in the Arabidopsis thaliana female gametophyte." Plant reproduction 30, 3 (September 2017): 141–146 © 2017, The Author(s).
Version
Final published version
Abstract
Cellular decisions can be influenced by information communicated from neighboring cells. Communication can occur via signaling or through the direct transfer of molecules. Movement of RNAs and proteins has frequently been observed among symplastically connected plant cells. In flowering plants, the female gametes, the egg cell and central cell, are closely apposed within the female gametophyte. Here we investigated the ability of fluorescently labeled dyes and small RNAs to move from the Arabidopsis thaliana central cell to the egg apparatus following microinjection. These results define a size limit of at least 20 kDa for symplastic movement between the two gametes, somewhat larger than that previously observed in Torenia fournieri. Our results indicate that symplastic connectivity in Arabidopsis thaliana changes after fertilization and suggest that prior to fertilization mechanisms are in place to facilitate small RNA movement from the central cell to the egg cell and synergids. Keywords: Female gametophyte; Central cell; Egg apparatus; Symplastic movement; Small RNAs; Arabidopsis thaliana.
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/s00497-017-0304-3
