Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis
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Plasma membrane.pdf
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Author(s)
Kotýnková, Kristýna
Su, Kuan-Chung
West, Stephen C.
Petronczki, Mark
Date Issued
December 2016
Journal
Cell Reports
Publisher
Elsevier
Citation
Kotýnková, Kristýna, Kuan-Chung Su, Stephen C. West, and Mark Petronczki. “Plasma Membrane Association but Not Midzone Recruitment of RhoGEF ECT2 Is Essential for Cytokinesis.” Cell Reports 17, no. 10 (December 2016): 2672–2686.
Version
Final published version
Abstract
Cytokinesis, the final step of cell division, begins with the formation of a cleavage furrow. How the mitotic spindle specifies the furrow at the equator in animal cells remains unknown. Current models propose that the concentration of the RhoGEF ECT2 at the spindle midzone and the equatorial plasma membrane directs furrow formation. Using chemical genetic and optogenetic tools, we demonstrate that the association of ECT2 with the plasma membrane during anaphase is required and sufficient for cytokinesis. Local membrane targeting of ECT2 leads to unilateral furrowing, highlighting the importance of local ECT2 activity. ECT2 mutations that prevent centralspindlin binding compromise concentration of ECT2 at the midzone and equatorial membrane but sustain cytokinesis. While the association of ECT2 with the plasma membrane is essential for cytokinesis, our data suggest that ECT2 recruitment to the spindle midzone is insufficient to account for equatorial furrowing and may act redundantly with yet-uncharacterized signals.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2016.11.029
