Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
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Author(s)
Bryson, Bryan D
Butler, Andrew
Satija, Rahul
Fortune, Sarah
Love, J Christopher
Gierahn, Todd Michael
Wadsworth, Marc Havens
Hughes, Travis K.
Shalek, Alexander K
Date Issued
February 2017
Journal
Nature Methods
Publisher
Springer Nature
Citation
Gierahn, Todd M et al. “Seq-Well: Portable, Low-Cost RNA Sequencing of Single Cells at High Throughput.” Nature Methods 14, 4 (February 2017): 395–398 © 2017 KSBMB
Version
Author's final manuscript
Abstract
Single-cell rna-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. here, we present seq-Well, a portable, low-cost platform for massively parallel single-cell rna-seq. barcoded mrna capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling effcient cell lysis and transcript capture. We use seq-Well to profle thousands of primary human macrophages exposed to Mycobacterium tuberculosis.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/NMETH.4179
