Locating and Characterizing a Transgene Integration Site by Nanopore Sequencing
Name
1481.full.pdf
Description
Published version
Size
1.08 MB
Format
Adobe PDF
Checksum (MD5)
b7a4111fddf91c3b86d3352f6faff63a
Author(s)
Nicholls, Peter K.
Bellott, Daniel W.
Cho, Ting-Jan
Pyntikova, Tatyana
Page, David C.
Date Issued
May 2019
Journal
G3
Publisher
Genetics Society of America
Citation
Nicholls, Peter K. et al. “Locating and Characterizing a Transgene Integration Site by Nanopore Sequencing.” G3 9 (2019): 1481-1486 © 2019 The Author(s)
Version
Final published version
Abstract
The introduction of foreign DNA into cells and organisms has facilitated much of modern biological research, and it promises to become equally important in clinical practice. Locating sites of foreign DNA incorporation in mammalian genomes has proven burdensome, so the genomic location of most transgenes remains unknown. To address this challenge, we applied nanopore sequencing in search of the site of integration of Tg(Pou5f1-EGFP)2Mnn (also known as Oct4:EGFP), a widely used fluorescent reporter in mouse germ line research. Using this nanopore-based approach, we identified the site of Oct4: EGFP transgene integration near the telomere of Chromosome 9. This methodology simultaneously yielded an estimate of transgene copy number, provided direct evidence of transgene inversions, revealed contaminating E. coli genomic DNA within the transgene array, validated the integrity of neighboring genes, and enabled definitive genotyping. We suggest that such an approach provides a rapid, cost-effective method for identifying and analyzing transgene integration sites.
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1534/G3.119.300582
