DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes
Name
Chareonsawan_2012-DNA sequence preferences.pdf
Size
1.18 MB
Format
Adobe PDF
Checksum (MD5)
5f0022fe58b78411363d01df7ea0fbb4
Author(s)
Charoensawan, Varodom
Janga, Sarath Chandra
Bulyk, Martha L.
Babu, M. Madan
Teichmann, Sarah A.
Date Issued
July 2012
Journal
Molecular Cell
Publisher
Elsevier
Citation
Charoensawan, Varodom, Sarath Chandra Janga, Martha L. Bulyk, M. Madan Babu, and Sarah A. Teichmann. “DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes.” Molecular Cell 47, no. 2 (July 2012): 183-192. © 2012 Elsevier Inc.
Version
Final published version
Abstract
Transcription factors (TFs) and histone octamers are two abundant classes of DNA binding proteins that coordinate the transcriptional program in cells. Detailed studies of individual TFs have shown that TFs bind to nucleosome-occluded DNA sequences and induce nucleosome disruption/repositioning, while recent global studies suggest this is not the only mechanism used by all TFs. We have analyzed to what extent the intrinsic DNA binding preferences of TFs and histones play a role in determining nucleosome occupancy, in addition to nonintrinsic factors such as the enzymatic activity of chromatin remodelers. The majority of TFs in budding yeast have an intrinsic sequence preference overlapping with nucleosomal histones. TFs with intrinsic DNA binding properties highly correlated with those of histones tend to be associated with gene activation and might compete with histones to bind to genomic DNA. Consistent with this, we show that activators induce more nucleosome disruption upon transcriptional activation than repressors.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Article is available under a Creative Commons license.
Persistent DSpace Link
DOI of Published Version
http://dx.doi.org/10.1016/j.molcel.2012.06.028
