Iterative expansion microscopy using lipid and protein labels for nano-scale imaging of brain circuits
Name
1227278588-MIT.pdf
Size
1.07 MB
Format
Adobe PDF
Checksum (MD5)
ab9b9db07d374eacc5bc7b89a31b3c04
Author(s)
Shin, Tay Won.
Advisor(s)
Edward Boyden.
Alternative Title
Iterative ExM using lipid and protein labels for nano-scale imaging of brain circuits
Date Issued
2020
Publisher
Massachusetts Institute of Technology
Abstract
Expansion microscopy(ExM) and iterative expansion microscopy(iExM) enables nanometer resolution by physically expanding fixed specimens using a conventional diffraction limited optical microscope. Here, we introduce a polymer-anchorable lipid intercalating label that binds to membranes of fixed tissue to perform ExM and iExM, which we call membrane expansion microscopy (mExM) and membrane iterative expansion microscopy (imExM), respectively. The technology is also compatible with immunohistochemistry to visualize proteins of interest (i.e. nuclear pore complex, Golgi apparatus, etc.). With the technology, we can reconstruct structural and molecular information at a resolution between approximately 15nm to 20 nm.
Description
Thesis: S.M., Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, September, 2020
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 35-39).
Subjects
Program in Media Arts and Sciences
MIT Department
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
MIT theses may be protected by copyright. Please reuse MIT thesis content according to the MIT Libraries Permissions Policy, which is available through the URL provided.
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