Metabolic remodeling of the human red blood cell membrane
Name
Park-2010-Metabolic remodeling.pdf
Size
989.5 KB
Format
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Checksum (MD5)
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Author(s)
Suresh, Subra
Park, YongKeun
Best, Catherine A.
Auth, Thorsten
Gov, Nir S.
Safan, Samuel A.
Popescu, Gabriel
Feld, Michael S.
Date Issued
January 2010
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences
Citation
Park, YongKeun et al. “Metabolic remodeling of the human red blood cell membrane.” Proceedings of the National Academy of Sciences 107.4 (2010): 1289 -1294. ©2010 by the National Academy of Sciences.
Version
Final published version
Abstract
The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. Here we present quantitative connections between spectrin morphology and membrane fluctuations of human RBCs by using dynamic full-field laser interferometry techniques. We present conclusive evidence that the presence of adenosine 5′-triphosphate (ATP) facilitates non-equilibrium dynamic fluctuations in the RBC membrane that are highly correlated with the biconcave shape of RBCs. Spatial analysis of the fluctuations reveals that these non-equilibrium membrane vibrations are enhanced at the scale of spectrin mesh size. Our results indicate that the dynamic remodeling of the coupled membranes powered by ATP results in non-equilibrium membrane fluctuations manifesting from both metabolic and thermal energies and also maintains the biconcave shape of RBCs.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Spectroscopy Laboratory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.0910785107
