Cell swelling, softening and invasion in a three-dimensional breast cancer model
Name
Han et al Nature Physical-final proof.pdf
Description
Accepted version
Size
1.23 MB
Format
Adobe PDF
Checksum (MD5)
707010aaae46556ca8c1c8f3e5c5a9c0
Author(s)
Han, Yulong
Pegoraro, Adrian F.
Li, Hui
Li, Kaifu
Yuan, Yuan
Xu, Guoqiang
Gu, Zichen
Sun, Jiawei
Hao, Yukun
Gupta, Satish Kumar
Date Issued
October 2019
Journal
Nature Physics
Publisher
Springer
Citation
Han, Yu Long et al. "Cell swelling, softening and invasion in a three-dimensional breast cancer model." Nature Physics 16, 1 (October 2019): 101–108 © 2019 The Author(s)
Version
Author's final manuscript
Abstract
Control of the structure and function of three-dimensional multicellular tissues depends critically on the spatial and temporal coordination of cellular physical properties, yet the organizational principles that govern these events and their disruption in disease remain poorly understood. Using a multicellular mammary cancer organoid model, we map here the spatial and temporal evolution of positions, motions and physical characteristics of individual cells in three dimensions. Compared with cells in the organoid core, cells at the organoid periphery and the invasive front are found to be systematically softer, larger and more dynamic. These mechanical changes are shown to arise from supracellular fluid flow through gap junctions, the suppression of which delays the transition to an invasive phenotype. These findings highlight the role of spatiotemporal coordination of cellular physical properties in tissue organization and disease progression.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41567-019-0680-8
