The protein phosphatase 2A functions in the spindle position checkpoint by regulating the checkpoint kinase Kin4
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manuscript-small.pdf
Description
Manuscript
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1.18 MB
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Adobe PDF
Checksum (MD5)
9658dd13fa6d34c09d98d61911699029
Author(s)
Chan, Leon Y.
Amon, Angelika B
Date Issued
July 2009
Journal
Genes & Development
Publisher
Cold Spring Harbor Laboratory Press
Citation
Chan, L. Y., and A. Amon. “The Protein Phosphatase 2A Functions in the Spindle Position Checkpoint by Regulating the Checkpoint Kinase Kin4.” Genes & Development 23.14 (2009): 1639–1649. CrossRef. Web.
Version
Author's final manuscript
Abstract
In budding yeast, a surveillance mechanism known as the spindle position checkpoint (SPOC) ensures accurate genome partitioning. In the event of spindle misposition, the checkpoint delays exit from mitosis by restraining the activity of the mitotic exit network (MEN). To date, the only component of the checkpoint to be identified is the protein kinase Kin4. Furthermore, how the kinase is regulated by spindle position is not known. Here, we identify the protein phosphatase 2A (PP2A) in complex with the regulatory subunit Rts1 as a component of the SPOC. Loss of PP2A-Rts1 function abrogates the SPOC but not other mitotic checkpoints. We further show that the protein phosphatase functions upstream of Kin4, regulating the kinase's phosphorylation and localization during an unperturbed cell cycle and during SPOC activation, thus defining the phosphatase as a key regulator of SPOC function.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1101/gad.1804609
