Promoters Recognized by Forkhead Proteins Exist for Individual 21U-RNAs
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Cecere-2012-Promoters Recognized.pdf
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Author(s)
Cecere, Germano
Zheng, Grace X.Y.
Mansisidor, Andres R.
Klymko, Katherine E.
Grishok, Alla
Date Issued
July 2012
Journal
Molecular Cell
Publisher
Elsevier
Citation
Cecere, Germano, Grace X.Y. Zheng, Andres R. Mansisidor, Katherine E. Klymko, and Alla Grishok. “Promoters Recognized by Forkhead Proteins Exist for Individual 21U-RNAs.” Molecular Cell 47, no. 5 (September 2012): 734–745. © 2012 Elsevier Inc.
Version
Final published version
Abstract
C. elegans 21U-RNAs are equivalent to the piRNAs discovered in other metazoans and have important roles in gametogenesis and transposon control. The biogenesis and molecular function of 21U-RNAs and piRNAs are poorly understood. Here, we demonstrate that transcription of each 21U-RNA is regulated separately through a conserved upstream DNA motif. We use genomic analysis to show that this motif is associated with low nucleosome occupancy, a characteristic of many promoters that drive expression of protein-coding genes, and that RNA polymerase II is localized to this nucleosome-depleted region. We establish that the most conserved 8-mer sequence in the upstream region of 21U-RNAs, CTGTTTCA, is absolutely required for their individual expression. Furthermore, we demonstrate that the 8-mer is specifically recognized by Forkhead family (FKH) transcription factors and that 21U-RNA expression is diminished in several FKH mutants. Our results suggest that thousands of small noncoding transcription units are regulated by FKH proteins.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2012.06.021
