Sub-membrane actin rings compartmentalize the plasma membrane
Name
jcb_202310138.pdf
Description
Published version
Size
7.68 MB
Format
Adobe PDF
Checksum (MD5)
f04dff44fc1e5d510374cbec47405772
Author(s)
Rentsch, Jakob
Bandstra, Selle
Sezen, Batuhan
Sigrist, Philipp
Bottanelli, Francesca
Schmerl, Bettina
Shoichet, Sarah
Noé, Frank
Sadeghi, Mohsen
Ewers, Helge
Date Issued
April 1, 2024
Journal
Journal of Cell Biology
Publisher
Rockefeller University Press
Citation
Jakob Rentsch, Selle Bandstra, Batuhan Sezen, Philipp Sigrist, Francesca Bottanelli, Bettina Schmerl, Sarah Shoichet, Frank Noé, Mohsen Sadeghi, Helge Ewers; Sub-membrane actin rings compartmentalize the plasma membrane. J Cell Biol 1 April 2024; 223 (4): e202310138.
Version
Final published version
Abstract
The compartmentalization of the plasma membrane (PM) is a fundamental feature of cells. The diffusivity of membrane proteins is significantly lower in biological than in artificial membranes. This is likely due to actin filaments, but assays to prove a direct dependence remain elusive. We recently showed that periodic actin rings in the neuronal axon initial segment (AIS) confine membrane protein motion between them. Still, the local enrichment of ion channels offers an alternative explanation. Here we show, using computational modeling, that in contrast to actin rings, ion channels in the AIS cannot mediate confinement. Furthermore, we show, employing a combinatorial approach of single particle tracking and super-resolution microscopy, that actin rings are close to the PM and that they confine membrane proteins in several neuronal cell types. Finally, we show that actin disruption leads to loss of compartmentalization. Taken together, we here develop a system for the investigation of membrane compartmentalization and show that actin rings compartmentalize the PM.
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Creative Commons Attribution-NonCommercial-ShareAlike
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DOI of Published Version
https://doi.org/10.1083/jcb.202310138
