This is not the latest version of this item. The latest version can be found here.
Quantification of human neuromuscular function through optogenetics
Name
v09p1232.pdf
Description
Published version
Size
1.88 MB
Format
Adobe PDF
Checksum (MD5)
83e7b4c5e65e0977728c4e46c902e82b
Author(s)
Vila, Olaia F
Uzel, Sebastien GM
Ma, Stephen P
Williams, Damian
Pak, Joseph
Kamm, Roger D
Vunjak-Novakovic, Gordana
Date Issued
2019
Journal
Theranostics
Publisher
Ivyspring International Publisher
Version
Final published version
Abstract
© Ivyspring International Publisher. The study of human neuromuscular diseases has traditionally been performed in animal models, due to the difficulty of performing studies in human subjects. Despite the unquestioned value of animal models, inter-species differences hamper the translation of these findings to clinical trials. Tissue-engineered models of the neuromuscular junction (NMJ) allow for the recapitulation of the human physiology in tightly controlled in vitro settings. Methods: Here we report the first human patient-specific tissue-engineered model of the neuromuscular junction (NMJ) that combines stem cell technology with tissue engineering, optogenetics, microfabrication and image processing. The combination of custom-made hardware and software allows for repeated, quantitative measurements of NMJ function in a user-independent manner. Results: We demonstrate the utility of this model for basic and translational research by characterizing in real time the functional changes during physiological and pathological processes. Principal Conclusions: This system holds great potential for the study of neuromuscular diseases and drug screening, allowing for the extraction of quantitative functional data from a human, patient-specific system.
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
Persistent DSpace Link
DOI of Published Version
10.7150/THNO.25735
