Genetic encoding of DNA nanostructures and their self-assembly in living bacteria
Name
Elbaz-2016-Genetic encoding.pdf
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1.39 MB
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Adobe PDF
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Author(s)
Elbaz, Johann
Yin, Peng
Voigt, Christopher A.
Date Issued
April 2016
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Elbaz, Johann, Peng Yin, and Christopher A. Voigt. “Genetic Encoding of DNA Nanostructures and Their Self-Assembly in Living Bacteria.” Nat Comms 7 (April 19, 2016): 11179.
Version
Final published version
Abstract
The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32–205 nt), which can form structures in vivo or be purified for in vitro assembly. Each ssDNA is encoded by a gene that is transcribed into non-coding RNA containing a 3′-hairpin (HTBS). HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is produced in vivo is used to assemble large 1D wires (300 nm) and 2D sheets (5.8 μm2) in vitro. Intracellular assembly is demonstrated using a four-ssDNA crossover nanostructure that recruits split YFP when properly assembled. Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells.
MIT Department
Massachusetts Institute of Technology. Synthetic Biology Center
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Synthetic Biology Center
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1038/ncomms11179
