Design and Evolution of Engineered Biological Systems
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JK_ICSB_2005.pdf
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254.96 KB
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Adobe PDF
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Author(s)
Kelly, Jason
Michener, Josh
Endy, Drew
Date Issued
October 20, 2005
Publisher
Sixth International Conference on Systems Biology
Abstract
To date, engineered biological systems have been constructed via a variety of
ad hoc approaches. The resulting systems should be thought of as pieces of
art. We are interested in exploring how existing forward engineering
approaches might be combined with directed evolution to make routine the
construction of engineered biological systems. We have specified a
procedure for construction of biological systems via screening of
subcomponent libraries and rational re-assembly. We have begun
development of tools to enable this approach, including a FACS-based
screening system to rapidly measure the input/output function of a genetic
circuit. Additionally, we have designed a microfluidic system that enables
more sophisticated screening and selection functions. Specifically, a
microfluidic chemostat integrated with a cell sorter (i.e., a sortostat). This
microscope-based system will enable us to evaluate whether or not more
complicated screens and selections will be of practical use in service of
evolving engineered biological systems.
Description
Poster presented at the 2005 ICSB meeting, held at Harvard Medical School in Boston, MA.
Subjects
evolution
design
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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