A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsW
Name
GENESDEV-2011-164046v2-Laub-2[1].pdf
Description
main article
Size
7.18 MB
Format
Adobe PDF
Checksum (MD5)
6f4942e7b9581135342f0df44ccb3c56
Author(s)
Modell, Joshua W.
Hopkins, Alexander C.
Laub, Michael T.
Date Issued
May 2011
Journal
Genes and Development
Publisher
Cold Spring Harbor Laboratory
Citation
Modell, J. W., A. C. Hopkins, and M. T. Laub. “A DNA Damage Checkpoint in Caulobacter Crescentus Inhibits Cell Division Through a Direct Interaction with FtsW.” Genes & Development 25.12 (2011): 1328–1343. Web.
Version
Author's final manuscript
Abstract
Following DNA damage, cells typically delay cell cycle progression and inhibit cell division until their chromosomes have been repaired. The bacterial checkpoint systems responsible for these DNA damage responses are incompletely understood. Here, we show that Caulobacter crescentus responds to DNA damage by coordinately inducing an SOS regulon and inhibiting the master regulator CtrA. Included in the SOS regulon is sidA (SOS-induced inhibitor of cell division A), a membrane protein of only 29 amino acids that helps to delay cell division following DNA damage, but is dispensable in undamaged cells. SidA is sufficient, when overproduced, to block cell division. However, unlike many other regulators of bacterial cell division, SidA does not directly disrupt the assembly or stability of the cytokinetic ring protein FtsZ, nor does it affect the recruitment of other components of the cell division machinery. Instead, we provide evidence that SidA inhibits division by binding directly to FtsW to prevent the final constriction of the cytokinetic ring.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1101/gad.2038911
