Selective DNA-Directed Assembly on Dual-Functionalized Microparticles
Name
MEBCS023.pdf
Size
360.97 KB
Format
Adobe PDF
Checksum (MD5)
8302a2f9814c2470987162ceee65005c
Author(s)
Bajaj, Manish G.
Laibinis, Paul E.
Date Issued
January 2004
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS);
Abstract
The bottom-up assembly of functional devices requires novel building blocks to facilitate the incorporation of functional and structural hierarchy. Anisotropic building blocks can substantially broaden the creation of self-assembled devices with unique properties because of their morphological and/or chemical asymmetry. In this regard, we have created microspheres with one hemispherical face exposing silica and the other exposing gold. These microspheres were formed by the shadow deposition of gold onto silica microspheres. The two chemical surfaces allowed use of different surface reactions—silane chemistry for the silica side and thiol chemistry for the gold side—for immobilizing different oligonucleotide sequences on each of the two faces. These dual-functionalized microspheres were used in the selective orthogonal assembly of fluorophore-tagged target oligonucleotides. The DNA-directed assembly was confirmed by confocal microscopy of the microspheres. In essence, employing DNA as the linker molecule, these “Janus” particles can be assembled into various novel 1-D, 2-D, and 3-D structures, which are difficult to realize using symmetric building blocks.
Subjects
DNA attachment
directed-assembly microparticles
bifunctionality
Persistent DSpace Link
