Skip to main content
We are experiencing minor difficulties with our submission forms, which we are working to resolve. If you have any issues while submitting items, please contact us at dspace-lib@mit.edu.
Log in
(current)
MIT Open Scholarship
DSpace@MIT
Search
Home
MIT Open Access Articles
MIT Open Access Articles
High-sensitivity protein solid-state NMR spectroscopy
This is not the latest version of this item. The latest version can be found
here
.
High-sensitivity protein solid-state NMR spectroscopy
Download
Name
nihms-1526024.pdf
Description
Accepted version
Show more
Size
581.27 KB
Format
Adobe PDF
Checksum
(MD5)
59ffb859482081e92c9a07420e60bb38
Show more
sword-2020-10-19T14:25:42.original.xml (130 B)
Original SWORD entry document
Show more
Author(s)
Mandala, Venkata S
•
Hong, Mei
Journal
Current Opinion in Structural Biology
Publisher
Elsevier BV
Version
Author's final manuscript
Abstract
© 2019 Elsevier Ltd The sensitivity of solid-state nuclear magnetic resonance (SSNMR) spectroscopy for structural biology is significantly increased by 1H detection under fast magic-angle spinning (MAS) and by dynamic nuclear polarization (DNP) from electron spins to nuclear spins. The former allows studies of the structure and dynamics of small quantities of proteins under physiological conditions, while the latter permits studies of large biomolecular complexes in lipid membranes and cells, protein intermediates, and protein conformational distributions. We highlight recent applications of these two emerging SSNMR technologies and point out areas for future development.
Show more
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
http://creativecommons.org/licenses/by-nc-nd/4.0/
Persistent DSpace Link
https://hdl.handle.net/1721.1/132388
DOI of Published Version
10.1016/J.SBI.2019.03.027
