Photodynamic Therapy Can Induce a Protective Innate Immune Response against Murine Bacterial Arthritis via Neutrophil Accumulation
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Tanaka-2012-Photodynamic Therapy.pdf
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4.72 MB
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Author(s)
Tanaka, Masamitsu
Mroz, Pawel
Dai, Tianhong
Huang, Liyi
Morimoto, Yuji
Kinoshita, Manabu
Yoshihara, Yasuo
Nemoto, Koichi
Shinomiya, Nariyoshi
Seki, Suhji
Date Issued
June 2012
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Tanaka, Masamitsu et al. “Photodynamic Therapy Can Induce a Protective Innate Immune Response Against Murine Bacterial Arthritis via Neutrophil Accumulation.” Ed. Hossam M. Ashour. PLoS ONE 7.6 (2012): e39823.
Version
Final published version
Abstract
Background:
Local microbial infections induced by multiple-drug-resistant bacteria in the orthopedic field can be intractable, therefore development of new therapeutic modalities is needed. Photodynamic therapy (PDT) is a promising alternative modality to antibiotics for intractable microbial infections, and we recently reported that PDT has the potential to accumulate neutrophils into the infected site which leads to resolution of the infection. PDT for cancer has long been known to be able to stimulate the innate and adaptive arms of the immune system.
Methodology/Principal Findings:
In the present study, a murine methicillin-resistant Staphylococcus aureus (MRSA) arthritis model using bioluminescent MRSA and polystyrene microparticles was established, and both the therapeutic (Th-PDT) and preventive (Pre-PDT) effects of PDT using methylene blue as photosensitizer were examined. Although Th-PDT could not demonstrate direct bacterial killing, neutrophils were accumulated into the infectious joint space after PDT and MRSA arthritis was reduced. With the preconditioning Pre-PDT regimen, neutrophils were quickly accumulated into the joint immediately after bacterial inoculation and bacterial growth was suppressed and the establishment of infection was inhibited.
Conclusions/Significance:
This is the first demonstration of a protective innate immune response against a bacterial pathogen produced by PDT.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0039823
