Sub-population analysis based on temporal features of high content images
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Veronika-2009-Sub-population analy.pdf
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Author(s)
Rajapakse, Jagath
Matsudaira, Paul T.
Evans, James
Welsch, Roy E.
Veronika, Merlin
Date Issued
December 2009
Journal
BMC Bioinformatics
Publisher
BioMed Central
Citation
Veronika, Merlin et al. “Sub-population analysis based on temporal features of high content images.” BMC Bioinformatics 10.Suppl 15 (2009): S4.
Version
Final published version
Abstract
Background: High content screening techniques are increasingly used to understand the regulation and progression of cell motility. The demand of new platforms, coupled with availability of terabytes of data has challenged the traditional technique of identifying cell populations by manual methods and resulted in development of high-dimensional analytical methods.
Results: In this paper, we present sub-populations analysis of cells at the tissue level by using dynamic features of the cells. We used active contour without edges for segmentation of cells, which preserves the cell morphology, and autoregressive modeling to model cell trajectories. The sub-populations were obtained by clustering static, dynamic and a combination of both features. We were able to identify three unique sub-populations in combined clustering.
Conclusion: We report a novel method to identify sub-populations using kinetic features and demonstrate that these features improve sub-population analysis at the tissue level. These advances will facilitate the application of high content screening data analysis to new and complex biological problems.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Sloan School of Management
Whitehead Institute for Biomedical Research
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Creative Commons Attribution
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DOI of Published Version
http://dx.doi.org/10.1186/1471-2105-10-s15-s4
