Cellular-scale probes enable stable chronic subsecond monitoring of dopamine neurochemicals in a rodent model
Name
s42003-018-0147-y.pdf
Description
Published version
Size
1.8 MB
Format
Adobe PDF
Checksum (MD5)
72f893e7d99479a50c75308cef009875
Author(s)
Schwerdt, Helen N
Zhang, Elizabeth
Kim, Min Jung
Yoshida, Tomoko
Stanwicks, Lauren
Amemori, Satoko
Dagdeviren, Huseyin E
Langer, Robert
Cima, Michael J
Graybiel, Ann M
Date Issued
2018
Journal
Communications Biology
Publisher
Springer Nature
Version
Final published version
Abstract
© 2018, The Author(s). Chemical signaling underlies both temporally phasic and extended activity in the brain. Phasic activity can be monitored by implanted sensors, but chronic recording of such chemical signals has been difficult because the capacity to measure them degrades over time. This degradation has been attributed to tissue damage progressively produced by the sensors and failure of the sensors themselves. We report methods that surmount these problems through the development of sensors having diameters as small as individual neuronal cell bodies (<10 µm). These micro-invasive probes (µIPs) markedly reduced expression of detectable markers of inflammation and tissue damage in a rodent test model. The chronically implanted µIPs provided stable operation in monitoring sub-second fluctuations in stimulation-evoked dopamine in anesthetized rats for over a year. These findings demonstrate that monitoring of chemical activity patterns in the brain over at least year-long periods, long a goal of both basic and clinical neuroscience, is achievable.
MIT Department
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/S42003-018-0147-Y
