Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease
Name
Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease
Size
839.84 KB
Format
Unknown
Checksum (MD5)
92fe325336be0b7963a9cf247904bc44
Author(s)
Bilate, Angelina M.
Huang, Nai-Jia
Li, Hojun
Patterson, Heide C.
Dougan, Stephanie K.
Maruyama, Takeshi
Pishesha, Novalia
Wibowo, Marsha C
Dhesycka, Rhogerry
Bousbaine, Djenet
Date Issued
March 2017
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Pishesha, Novalia et al. “Engineered Erythrocytes Covalently Linked to Antigenic Peptides Can Protect Against Autoimmune Disease.” Proceedings of the National Academy of Sciences 114, 12 (March 2017): 3157–3162 © 2017 National Academy of Sciences
Version
Final published version
Abstract
Current therapies for autoimmune diseases rely on traditional immunosuppressive medications that expose patients to an increased risk of opportunistic infections and other complications. Immunoregulatory interventions that act prophylactically or therapeutically to induce antigen-specific tolerance might overcome these obstacles. Here we use the transpeptidase sortase to covalently attach diseaseassociated autoantigens to genetically engineered and to unmodified red blood cells as a means of inducing antigen-specific tolerance. This approach blunts the contribution to immunity of major subsets of immune effector cells (B cells, CD4⁺ and CD8⁺ T cells) in an antigenspecific manner. Transfusion of red blood cells expressing self-antigen epitopes can alleviate and even prevent signs of disease in experimental autoimmune encephalomyelitis, as well as maintain normoglycemia in a mouse model of type 1 diabetes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/PNAS.1701746114
