Expansion microscopy : improving imaging through uniform tissue expansion
Name
965383260-MIT.pdf
Description
Full printable version
Size
9.24 MB
Format
Adobe PDF
Checksum (MD5)
37352ca009542bac49de3e06144ac1aa
Author(s)
Tillberg, Paul W
Advisor(s)
Edward S. Boyden.
Date Issued
2016
Publisher
Massachusetts Institute of Technology
Abstract
Until the past decade, optical microscopy of biological specimens was strongly limited by diffraction and scattering, affecting imaging resolution and depth, respectively. Now, numerous methods are available to overcome each of these limitations, but sub-diffraction limited resolution imaging over large volumes of scattering tissue is still a challenge. This work concerns the development of a new method, Expansion Microscopy (ExM) for achieving effect sub-diffraction-limited optical images in biological specimens. In ExM, the specimen is embedded in a swellable gel material to which fluorescent probes are chemically anchored. The embedded tissue is strongly digested so that it will not hinder uniform expansion driven by the gel. The gel with embedded, fragmented tissue is washed in water, triggering expansion of around 4-fold in each dimension. A variant of the method, ExM with Protein Retention (proExM) is presented that allows proteins themselves, rather than fluorescent probes, to be anchored by a small molecule cross-linker to the gel, so that the method may be carried out entirely with commercial components and standard antibodies.
Description
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2016.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 70-76).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
Persistent DSpace Link
