Photoelectrochemical array platform for genomic scale DNA synthesis
Name
78914934-MIT.pdf
Description
Full printable version
Size
14.62 MB
Format
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Checksum (MD5)
8b9934b6cdcd95ea607bea697fdff2e2
Author(s)
Emig, Christopher Joseph
Advisor(s)
Joseph M. Jacobson.
Date Issued
2006
Publisher
Massachusetts Institute of Technology
Abstract
Molecular and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled into large constructs of DNA for costs significantly less than traditional methods. This thesis presents a complete platform for the photoelectrochemical cleavage of protecting groups during in situ DNA synthesis. Photoelectrochemically patterned microarrays of phosphoramidite dye were produced with spot sizes of 100 um. This technology holds the potential for lowest cost per base pair of DNA produced over alternative microarray technologies, and may prove to be useful in de novo synthesis of large DNA constructs.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2006.
Includes bibliographical references (leaves 56-58).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
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