Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays
Name
BIOMGB-000012-024102_1.pdf
Description
Published version
Size
1.39 MB
Format
Adobe PDF
Checksum (MD5)
f9dbdfde1be4000a785b865c2586b0e6
Author(s)
Chen, Lynna
Kim, Jae Jung
Doyle, Patrick S
Date Issued
March 2018
Journal
Biomicrofluidics
Publisher
AIP Publishing
Citation
Chen, Lynna et al., "Microfluidic platform for selective microparticle parking and paired particle isolation in droplet arrays." Biomicrofluidics 12, 2 (March 2018): 024102 doi. 10.1063/1.5011342 ©2018 Author(s)
Version
Final published version
Abstract
Immobilizing microscale objects (e.g., cells, spheroids, and microparticles) in arrays for direct observation and analysis is a critical step of many biological and chemical assays; however, existing techniques are often limited in their ability to precisely capture, arrange, isolate, and recollect objects of interest. In this work, we present a microfluidic platform that selectively parks microparticles in hydrodynamic traps based on particle physical characteristics (size, stiffness, and internal structure). We present an accompanying scaling analysis for the particle parking process to enable rational design of microfluidic traps and selection of operating conditions for successful parking of desired particles with specific size and elastic modulus. Our platform also enables parking of encoded particle pairs in defined spatial arrangements and subsequent isolation of these pairs in aqueous droplets, creating distinct microenvironments with no cross-contamination. In addition, we demonstrate the ability to recollect objects of interest (i.e., one particle from each pair) after observation within the channel. This integrated device is ideal for multiplexed assays or microenvironment fabrication for controlled biological studies. ©2018 Author(s).
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1063/1.5011342
