Genetic Determinants of Phosphate Response in Drosophila
Name
Bergwitz-2013-Genetic Determinants.pdf
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1.98 MB
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Author(s)
Bergwitz, Clemens
Wee, Mark J.
Sinha, Sumi
Huang, Joanne
DeRobertis, Charles
Mensah, Lawrence B.
Cohen, Jonathan
Friedman, Adam
Kulkarni, Meghana
Hu, Yanhui
Date Issued
March 2013
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Bergwitz, Clemens et al. “Genetic Determinants of Phosphate Response in Drosophila.” Ed. Fanis Missirlis. PLoS ONE 8.3 (2013): e56753.
Version
Final published version
Abstract
Phosphate is required for many important cellular processes and having too little phosphate or too much can cause disease and reduce life span in humans. However, the mechanisms underlying homeostatic control of extracellular phosphate levels and cellular effects of phosphate are poorly understood. Here, we establish Drosophila melanogaster as a model system for the study of phosphate effects. We found that Drosophila larval development depends on the availability of phosphate in the medium. Conversely, life span is reduced when adult flies are cultured on high phosphate medium or when hemolymph phosphate is increased in flies with impaired Malpighian tubules. In addition, RNAi-mediated inhibition of MAPK-signaling by knockdown of Ras85D, phl/D-Raf or Dsor1/MEK affects larval development, adult life span and hemolymph phosphate, suggesting that some in vivo effects involve activation of this signaling pathway by phosphate. To identify novel genetic determinants of phosphate responses, we used Drosophila hemocyte-like cultured cells (S2R+) to perform a genome-wide RNAi screen using MAPK activation as the readout. We identified a number of candidate genes potentially important for the cellular response to phosphate. Evaluation of 51 genes in live flies revealed some that affect larval development, adult life span and hemolymph phosphate levels.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Mathematics
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0056753
