Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication
Name
Ahadian-2014-Hybrid hydrogels.pdf
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1.49 MB
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Author(s)
Ahadian, Samad
Ramon-Azcon, Javier
Estili, Mehdi
Liang, Xiaobin
Ostrovidov, Serge
Shiku, Hitoshi
Ramalingam, Murugan
Nakajima, Ken
Sakka, Yoshio
Bae, Hojae
Date Issued
March 2014
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Ahadian, Samad, Javier Ramon-Azcon, Mehdi Estili, Xiaobin Liang, Serge Ostrovidov, Hitoshi Shiku, Murugan Ramalingam, et al. “Hybrid Hydrogels Containing Vertically Aligned Carbon Nanotubes with Anisotropic Electrical Conductivity for Muscle Myofiber Fabrication.” Sci. Rep. 4 (March 19, 2014).
Version
Final published version
Abstract
Biological scaffolds with tunable electrical and mechanical properties are of great interest in many different fields, such as regenerative medicine, biorobotics, and biosensing. In this study, dielectrophoresis (DEP) was used to vertically align carbon nanotubes (CNTs) within methacrylated gelatin (GelMA) hydrogels in a robust, simple, and rapid manner. GelMA-aligned CNT hydrogels showed anisotropic electrical conductivity and superior mechanical properties compared with pristine GelMA hydrogels and GelMA hydrogels containing randomly distributed CNTs. Skeletal muscle cells grown on vertically aligned CNTs in GelMA hydrogels yielded a higher number of functional myofibers than cells that were cultured on hydrogels with randomly distributed CNTs and horizontally aligned CNTs, as confirmed by the expression of myogenic genes and proteins. In addition, the myogenic gene and protein expression increased more profoundly after applying electrical stimulation along the direction of the aligned CNTs due to the anisotropic conductivity of the hybrid GelMA-vertically aligned CNT hydrogels. We believe that platform could attract great attention in other biomedical applications, such as biosensing, bioelectronics, and creating functional biomedical devices.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/srep04271
