Cellular normoxic biophysical markers of hydroxyurea treatment in sickle cell disease
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Author(s)
Hosseini, Poorya
Abidi, Sabia Z.
Du, E
Papageorgiou, Dimitrios P.
Choi, Youngwoon
Higgins, John M.
Kato, Gregory J.
Suresh, Subra
Dao, Ming
Yaqoob, Zahid
Date Issued
August 2016
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Hosseini, Poorya et al. “Cellular Normoxic Biophysical Markers of Hydroxyurea Treatment in Sickle Cell Disease.” Proceedings of the National Academy of Sciences 113, 34 (August 2016): 9527–9532 © 2016 National Academy of Sciences
Version
Final published version
Abstract
Hydroxyurea (HU) has been used clinically to reduce the frequency of painful crisis and the need for blood transfusion in sickle cell disease (SCD) patients. However, the mechanisms underlying such beneficial effects of HU treatment are still not fully understood. Studies have indicated a weak correlation between clinical outcome and molecular markers, and the scientific quest to develop companion biophysical markers have mostly targeted studies of blood properties under hypoxia. Using a common-path interferometric technique, we measure biomechanical and morphological properties of individual red blood cells in SCD patients as a function of cell density, and investigate the correlation of these biophysical properties with drug intake as well as other clinically measured parameters. Our results show that patient-specific HU effects on the cellular biophysical properties are detectable at normoxia, and that these properties are strongly correlated with the clinically measured mean cellular volume rather than fetal hemoglobin level.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/PNAS.1610435113
