Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-coding RNA
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Author(s)
Martinez-Moreno, Margot
Zepecki, John P.
Olaru, Alexander
Ness, Jennifer K.
Tapinos, Nikos
O'Shea, Timothy Mark
Langer, Robert S
Date Issued
August 2017
Journal
Cell Reports
Publisher
Elsevier
Citation
Martinez-Moreno, Margot et al. “Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-Coding RNA.” Cell Reports 20, 8 (August 2017): 1950–1963 © 2017 The Authors
Version
Final published version
Abstract
Precise regulation of Egr2 transcription is fundamentally important to the control of peripheral myelination. Here, we describe a long non-coding RNA antisense to the promoter of Egr2 (Egr2-AS-RNA). During peripheral nerve injury, the expression of Egr2-AS-RNA is increased and correlates with decreased Egr2 transcript and protein levels. Ectopic expression of Egr2-AS-RNA in dorsal root ganglion (DRG) cultures inhibits the expression of Egr2 mRNA and induces demyelination. In vivo inhibition of Egr2-AS-RNA using oligonucleotide GapMers released from a biodegradable hydrogel following sciatic nerve injury reverts the EGR2-mediated gene expression profile and significantly delays demyelination. Egr2-AS-RNA gradually recruits H3K27ME3, AGO1, AGO2, and EZH2 on the Egr2 promoter following sciatic nerve injury. Furthermore, expression of Egr2-AS-RNA is regulated through ERK1/2 signaling to YY1, while loss of Ser184 of YY1 regulates binding to Egr2-AS-RNA. In conclusion, we describe functional exploration of an antisense long non-coding RNA in peripheral nervous system (PNS) biology. Keywords: nerve injury response; transcription;
RNA epigenetics; antisense RNA; Egr2; myelination; YY1; neuregulin
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Koch Institute for Integrative Cancer Research at MIT
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Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
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DOI of Published Version
https://doi.org/10.1016/J.CELREP.2017.07.068
