Highly structured homolog pairing reflects functional organization of the Drosophila genome
Name
s41467-019-12208-3.pdf
Description
Published version
Size
4.43 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
AlHaj Abed, Jumana
Erceg, Jelena
Goloborodko, Anton
Nguyen, Son C.
McCole, Ruth B.
Saylor, Wren
Fudenberg, Geoffrey
Lajoie, Bryan R.
Dekker, Job
Mirny, Leonid A.
Date Issued
October 2019
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
AlHaj Abed, Jumana et al. "Highly structured homolog pairing reflects functional organization of the Drosophila genome." Nature Communications 10 (October 2019): 4485 ©2019 The Author(s)
Version
Final published version
Abstract
Trans-homolog interactions have been studied extensively in Drosophila, where homologs are paired in somatic cells and transvection is prevalent. Nevertheless, the detailed structure of pairing and its functional impact have not been thoroughly investigated. Accordingly, we generated a diploid cell line from divergent parents and applied haplotype-resolved Hi-C, showing that homologs pair with varying precision genome-wide, in addition to establishing trans-homolog domains and compartments. We also elucidate the structure of pairing with unprecedented detail, observing significant variation across the genome and revealing at least two forms of pairing: tight pairing, spanning contiguous small domains, and loose pairing, consisting of single larger domains. Strikingly, active genomic regions (A-type compartments, active chromatin, expressed genes) correlated with tight pairing, suggesting that pairing has a functional implication genome-wide. Finally, using RNAi and haplotype-resolved Hi-C, we show that disruption of pairing-promoting factors results in global changes in pairing, including the disruption of some interaction peaks. Keywords: Computational biology and bioinformatics; Epigenetics; Functional genomics; Molecular biology
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41467-019-12208-3
