SpiroZin1: A Reversible and pH-Insensitive, Reaction-Based, Red-Fluorescent Probe for Imaging Biological Mobile Zinc
Name
Lippard_SpiroZin1.pdf
Size
732.32 KB
Format
Adobe PDF
Checksum (MD5)
ebb30cafa7d4bf65569ebae00ab0e60a
Author(s)
Rivera-Fuentes, Pablo
Lippard, Stephen J.
Date Issued
March 2014
Journal
ChemMedChem
Publisher
Wiley Blackwell
Citation
Rivera-Fuentes, Pablo, and Stephen J. Lippard. “SpiroZin1: A Reversible and pH-Insensitive, Reaction-Based, Red-Fluorescent Probe for Imaging Biological Mobile Zinc.” ChemMedChem 9, no. 6 (March 11, 2014): 1238–1243.
Version
Author's final manuscript
Abstract
A reversible, reaction-based sensor for biological mobile zinc was designed, prepared, and characterized. The sensing mechanism of this probe is based on the zinc-induced, ring-opening reaction of spirobenzopyran to give a cyanine fluorophore that emits in the deep-red region of the electromagnetic spectrum. This probe is not activated by protons and operates efficiently in aqueous solution at pH 7 and high ionic strength. The mechanism of this reaction was studied by using a combination of kinetics experiments and DFT calculations. The biocompatibility of the probe was demonstrated in live HeLa cells.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/cmdc.201400014
