Synthesis and patterning of tunable multiscale materials with engineered cells
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Lu_Synthesis and.pdf
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Author(s)
Chen, Allen Y.
Deng, Zhengtao
Billings, Amanda N.
Seker, Urartu O. S.
Lu, Michelle Y.
Citorik, Robert James
Zakeri, Bijan
Lu, Timothy K.
Date Issued
March 2014
Journal
Nature Materials
Publisher
Nature Publishing Group
Citation
Chen, Allen Y., Zhengtao Deng, Amanda N. Billings, Urartu O. S. Seker, Michelle Y. Lu, Robert J. Citorik, Bijan Zakeri, and Timothy K. Lu. “Synthesis and Patterning of Tunable Multiscale Materials with Engineered Cells.” Nat Mater 13, no. 5 (March 23, 2014): 515–523.
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Author's final manuscript
Abstract
Many natural biological systems—such as biofilms, shells and skeletal tissues—are able to assemble multifunctional and environmentally responsive multiscale assemblies of living and non-living components. Here, by using inducible genetic circuits and cellular communication circuits to regulate Escherichia coli curli amyloid production, we show that E. coli cells can organize self-assembling amyloid fibrils across multiple length scales, producing amyloid-based materials that are either externally controllable or undergo autonomous patterning. We also interfaced curli fibrils with inorganic materials, such as gold nanoparticles (AuNPs) and quantum dots (QDs), and used these capabilities to create an environmentally responsive biofilm-based electrical switch, produce gold nanowires and nanorods, co-localize AuNPs with CdTe/CdS QDs to modulate QD fluorescence lifetimes, and nucleate the formation of fluorescent ZnS QDs. This work lays a foundation for synthesizing, patterning, and controlling functional composite materials with engineered cells.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Microbiology Graduate Program
Massachusetts Institute of Technology. Research Laboratory of Electronics
Massachusetts Institute of Technology. Synthetic Biology Center
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmat3912
