Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling
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nihms954015.pdf
Description
Accepted version
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792.95 KB
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Author(s)
Myers, Samuel A.
Wright, Jason
Peckner, Ryan
Kalish, Brian T.
Zhang, Feng
Carr, Steven A
Date Issued
May 2018
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Meyers, Samuel A. et al. "Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling." Nature Methods 15, 6 (May 2018): 437–439 © 2018 The Author(s)
Version
Author's final manuscript
Abstract
Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.
MIT Department
Broad Institute of MIT and Harvard
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/s41592-018-0007-1
